Cloning and sequence analysis of LEAFY promoter from mango
2013
Luo, C. | He, X.H. | Tan, C. | Hu, Y. | Ou, S.J.
In order to study the rule of LEAFY gene expression and regulation in mango, three gene specific reverse primers were designed from the 5’ region of LFY cDNA sequence from mango and 1498 bp sequence was cloned from genomic DNA by chromosome walking method. In this sequence, 184 bp was cording sequence and deduced 61 amino acids, 1314 bp was promoter region sequence. The gene and promoter were 1498 bp in length (GenBank accession GU338039). Sequence analysis by PLACE and PlantCARE showed that LFY promoter contained the basic elements: TATA-box, CAAT-box, stress-induced elements and light-responsive elements, some other cis-acting elements involved in ABA-, GA-, CTK-, IAA- and sugar were also identified in the promoter. It is suggested that LFY gene may be regulated by sugar, hormone and light in mango. LFY promoter of mango has been successfully cloned, thus providing a certain basis for the application of the promoter to plant gene engineering.
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