Self-enhancement of GABA in rice bran using various stress treatments
2015
Kim, Hyun Soo | Lee, Eun Jung | Lim, Seung-Taik | Han, Jung-Ah
Gamma-aminobutyric acid (GABA) may be synthesized in plant tissues when the organism is under stressful conditions. Rice bran byproduct obtained from the milling of brown rice was treated under anaerobic storage with nitrogen at different temperatures (20–60°C) and moisture contents (10–50%) up to 12h. For the GABA synthesis, the storage at 30% moisture content and 40°C appeared optimal. Utilisation of an electrolyzed oxidizing water (EOW, pH 3.3) for moisture adjustment and addition of glutamic acid increased the GABA content in rice bran. The maximum GABA content in rice bran (523mg/100g) could be achieved by the anaerobic storage at 30% EOW for 5h at 40°C after an addition of glutamic acid (5mM). This amount was approximately 17 times higher than that in the control (30mg/100g). The use of EOW also prevented bacterial growth by decreasing the colony counts almost by half.
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