Supplementing Propionibacterium acidipropionici P169 does not affect methane production or volatile fatty acid profiles of different diets in in vitro rumen cultures from heifers
2014
Alazzeh, A. Y. | Smith, A. H. | Beauchemin, K. A. | Meale, S. J. | McAllister, T. A.
Effects of Propionibacterium acidipropionici P169 on gas, methane (CH ₄) production and volatile fatty acids in in vitro rumen cultures were studied. Ground barley (Experiment 1), mixed forage (Experiment 2) or mixed concentrate-forage (Experiment 3) served as substrates. Three doses of P169 were used in Experiments 1 and 2 [1.2 × 10 ⁴; 1.2 × 10 ⁶; 1.2 × 10 ⁸ colony forming unit (CFU)/mL], while two doses (2.4 × 10 ⁶; 2.4 × 10 ⁸ CFU/mL) were used in Experiment 3. In Experiment 1, P169 increased (P < 0.05) gas production at 6–12 h and CH ₄ (mL/g diet dry matter) at all times. In Experiment 2, CH ₄ was increased at 6 h (P < 0.01). At 12 h, propionate declined (P < 0.01) in Experiment 1 and increased at 48 h in Experiment 3. The inability of P169 to increase propionate and its lack of persistence in mixed ruminal cultures may account for its inability to alter CH ₄ production.
显示更多 [+] 显示较少 [-]