Dietary L‐arginine modulates immunosuppression in broilers inoculated with an intermediate strain of infectious bursa disease virus
2015
Tan, Jian‐Zhuang | Guo, Yu‐Ming | Applegate, Todd J | Du, En‐Cun | Zhao, Xu
BACKGROUND: The effects of dietary l‐arginine (Arg) on immunosuppression following infectious bursal disease virus (IBDV) inoculation in broiler chickens were evaluated. The design of this study was a 5 × 2 factorial arrangement (n = 5) with five Arg concentrations (starter: 9.9, 13.9, 17.6, 21.3 and 25.3 g kg⁻¹; grower–finisher: 9.5, 13.5, 17.1, 20.1 and 23.6 g kg⁻¹) with or without IBDV inoculation (IBDV or saline inoculation at 14 days). Chickens were sampled at 2, 4 and 6 days post‐inoculation (DPI) and 42 days of age. RESULTS: The IBDV inoculation decreased (P = 0.05) CD3⁺, CD4⁺, and CD8⁺T cell counts at 2 days post‐inoculation (DPI) and monocyte counts at 6 DPI; and reduced (P < 0.05) bursal interleukin‐1β (IL‐1β) mRNA expression at 2 DPI and serum IL‐6 concentration at 4 DPI. Increasing Arg concentration increased (P < 0.05) CD4⁺and CD8⁺T cell counts at 2 DPI, linearly increased (P = 0.05) CD3⁺T cell counts in IBDV‐inoculated groups and monocyte counts in control groups at 4 DPI; increased (P < 0.05) serum IL‐6 concentration in IBDV‐inoculated groups at 2 DPI; and increased (P < 0.05) serum anti‐IBDV antibody titres at 42 days of age. CONCLUSION: Varying concentrations of Arg supplementation attenuated IBDV inoculation induced immunosuppression via modulating circulating T cell sub‐populations. © 2014 Society of Chemical Industry
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