4-Coumarate: CoA Ligase Partitions Metabolites for Eugenol Biosynthesis
2013
Rastogi, Shubhra | Kumar, Ritesh | Chanotiya, Chandan S. | Shanker, Karuna | Gupta, Madan M. | Nagegowda, Dinesh A. | Shasany, Ajit K.
Biosynthesis of eugenol shares its initial steps with that of lignin, involving conversion of hydroxycinnamic acids to their corresponding coenzyme A (CoA) esters by 4-coumarate:CoA ligases (4CLs). In this investigation, a 4CL (OS4CL) was identified from glandular trichome-rich tissue of Ocimum sanctum with high sequence similarity to an isoform (OB4CL_ctg4) from Ocimum basilicum . The levels of OS4CL and OB4CL_ctg4 -like transcripts were highest in O. sanctum trichome, followed by leaf, stem and root. The eugenol content in leaf essential oil was positively correlated with the expression of OS4CL in the leaf at different developmental stages. Recombinant OS4CL showed the highest activity with p -coumaric acid, followed by ferulic, caffeic and trans -cinnamic acids. Transient RNA interference (RNAi) suppression of OS4CL in O. sanctum leaves caused a reduction in leaf eugenol content and trichome transcript level, with a considerable increase in endogenous p -coumaric, ferulic, trans -cinnamic and caffeic acids. A significant reduction in the expression levels was observed for OB4CL_ctg4 -related transcripts in suppressed trichome compared with transcripts similar to the other four isoforms (OB4CL_ctg1 , 2 , 3 and 5). Sinapic acid and lignin content were also unaffected in RNAi suppressed leaf samples. Transient expression of OS4CL–green fluorescent protein fusion protein in Arabidopsis protoplasts was associated with the cytosol. These results indicate metabolite channeling of intermediates towards eugenol by a specific 4CL and is the first report demonstrating the involvement of 4CL in creation of virtual compartments through substrate utilization and committing metabolites for eugenol biosynthesis at an early stage of the pathway.
显示更多 [+] 显示较少 [-]