Post-thaw motility and longevity of motility of imipramine-induced ejaculates of pony stallions
1994
McDonnell, S.M. | Turner, R.M.O.
Imipramine-induced ex copula ejaculates and fractionated in copula ejaculates were collected from each of 5 pony stallions for freezing in 5-ml straws, using a modified Kenney glucose skim-milk extender. Initial post-thaw total and progressive motilities and daily post-thaw total and progressive motilities, as well as the number of days to reach 0 progressively motile spermatozoa, were also similar for the 2 methods of collection. The percentage of morphologically normal spermatozoa both before freezing and after thawing were also similar for in copula and ex copula ejaculates. Consistent with previous work, imipramine-induced ejaculates were of extremely high sperm concentration and low volume compared with those of in copula ejaculates. In this study, imipramine-induced ejaculates were of significantly higher concentration of sperm and lower volume than fractionated (first 2-3 jets) in copula ejaculates. These results suggest that imipramine-induced ejaculates may be suitable for cryopreservation. Breeding trials are necessary to evaluate actual fertility of semen.
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