Effect of oxygen tension and serum during IVM on developmental competence of bovine oocytes
2010
Pereira, Michele M. | Machado, Marco A. | Costa, Fernanda Q. | Serapiao, Raquel V. | Viana, Joao H.M. | Camargo, Luiz S.A.
With an aim to improve the in vitro production of bovine embryos, the present study investigated the effect of serum and oxygen tension during IVM on oocyte developmental competence. Four experimental groups were evaluated: G1, 10% oestrus cow serum (OCS) with 20% O₂; G2, 0.1% polyvinyl alcohol (PVA) with 20% O₂; G3, 10% OCS with 5% O₂; and G4, 0.1% PVA with 5% O₂. The proportion of MII oocytes, blastocyst rates and total cell number were not affected (P > 0.05) when the OCS was replaced with PVA under 5% O₂, whereas a higher (P 0.05) were found in maturation and blastocyst rates. Significant differences were found in the amount of specific transcripts in oocytes matured under different conditions. In conclusion maturation with PVA and 5% O₂ provides an efficient in vitro culture condition for the maturation of bovine oocytes.
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