Antioxidant dietary fibre (ADF) in lipid rich fruits: adapted methodology for quantification and characterization
2012
Rufino, M.S.M. | Alves, R.E. | Brito, E.S. | Tabernero, M. | Perez-Jimenez, J. | Saura-Calixto, F.
Tropical fruits are rich sources of dietary fibre and associated phytochemicals, e.g., polyphenols. Some of them, such as palm (Arecaceae) fruits have high oil content. However, methodologies for the determination of dietary fibre were developed for foods with low oil content. Therefore, in this work we propose an alternative methodology to analyze the dietary fibre (DF) content of lipid-rich fruits, using Açaí palm (Euterpe oleraceae) as a model. As lipids may interfere with enzymatic activity, two strategies to avoid excess of fat during the analysis should be considered: i) include an enzymatic step with pancreatic lipase and bile porcine extract or ii) a sample preparation step using an organic solvent to remove the fat content. It was concluded that removing lipid content from sample by petroleum ether pretreatment may be more efficient than by lipase digestion. In this case, subsequent enzymatic treatment may be enhanced, increasing the release of polyphenols belonging to different classes. These observations should be confirmed with further studies.
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