Scytophycin production by axenic cultures of the Cyanobacterium Scytonema ocellatum
1994
Patterson, G.M.L. | Bolis, C.M.
Nonexenic clones prepared from the cyanobacterium Scytonema ocellatum Lyngbye ex Bornet et Flahault strain FF-66-3 exhibited a high degree of heterogeneity with respect to tolytoxin titer. Thirty-four of 114 clones (29.8%) isolated by fragmentation of Scytonema filaments did not produce detectable amounts of tolytoxin in culture. One clone (designated SO127) produced approximately twice as much tolytoxin as the parental culture and continued to produce tolytoxin after repeated subculture. Three axenic clones were prepared from SO127 by a combination of antibiotic treatment and mechanical separation. Although axenic cultures yielded slightly greater biomass, tolytoxin content did not significantly differ between axenic and nonexenic cultures, indicating that bacteria do not play a role in tolytoxin biosynthesis. Bacterial cultures derived from S. ocellatum did not produce detectable amounts of tolytoxin.
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