Single-cell-derived sibling lines are established as an experimental system to assess chromosome number variations in embryogenic callus cultures of sweet orange
2003
Hao, Y.J. | Deng, X.X.
Single-cell derived sibling lines of 'Anliucheng' sweet orange (Citrus sinensis (L.) Osbeck) were established with low-density protoplast culture. Cell densities of 1 to 5x10(4) cell·ml-1 were appropriate for this purpose, while a super-low cell density of 5x10(3) cell·cm l-1 made most protoplasts destined for programmed cell death (PCD) characterized by multi-nucleation and the formation of apoptotic bodies. These single-cell derived sibling lines were then used as an experimental system for genetic assessment. Cytological examination revealed that chromosome number variation arose spontaneously in the colony per se but not from pre-existing variation. Chromosome number variations continued after one year of subculture at 1-month intervals. However, the ploidy level of these cell lines remained relatively stable during the culture period. In addition, these chromosome number variations were selected against during plant regeneration process.
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