Photosynthetic changes in the inducible CAM plant Sedum telephium L. Following the imposition of water stress. II. Changes in the activity of phosphoenolpyruvate carboxylase
1988
Groenhof, A.C. | Bryant, J.A. | Etherington, J.R.
In Sedum telephium, the switch from a weak-CAM to a full-CAM mode of photosynthesis in response to water stress, is accompanied by a marked increase in the activity of the enzyme phosphoenolpyruate carboxylase (PEPC) during the dark period of a diurnal cycle. Fractionation of the enzyme by non-denaturing polyacrylamide gel electrophoresis gives two active species; the activity of the more mobile species increases with the switch into a full-CAM mode of photosynthesis. Fractionation of the enzyme by denaturing electrophoresis and by gel filtration indicates that the molecular species particularly active in CAM is a monomeric protein, whilst the other readily observable species is a dimer.
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