Combined antibacterial and antibiofilm activity of phenyllactic acid and bacteriocin XJS01 against Shigella flexneri
2022
Jiang, Yu-Hang | Yang, Lin-Yu | Xin, Wei-Gang | Zhang, Qi-Lin
This study investigated antibacterial and antibiofilm activity of the combined use of phenyllactic acid (PLA) and bacteriocin XJS01 against Shigella flexneri_14. The minimum inhibitory concentration (MIC) of PLA and XJS01 against S. flexneri_14 was 2.45 mg/mL and 18.75 μg/mL, respectively. Growth and kill kinetics assays showed that the combined use of 1/2MIC PLA plus 1/2MIC XJS01 had a better activity against planktonic S. flexneri_14 compared to treatment with PLA and XJS01 used singly (1/2MIC and 2MIC). Cellular biochemical and morphological analysis revealed the remarkable ability of the combination in disrupting cell appearance and promoting deformation of planktonic S. flexneri_14 compared to single use. Moreover, S. flexneri_14 biofilm formation was inhibited and degraded by the combination, which showed a more remarkable antibiofilm activity than PLA and XJS01 when used singly. This study demonstrates the synergistic antibacterial activity of PLA and XJS01 against S. flexneri_14 in either planktonic or biofilm states in foods.
显示更多 [+] 显示较少 [-]