Triplexes, duplexes, and homopurine complexes formed from homopurine and homopyrimidine DNA oligonucleotides
1990
Feigon, J. | Gilbert, D. | Rajagopal, P. | Wang, E. | Marel, G.A. van der | Boom, J.H. van
Homopurine:homopyrimidine sequences are widely occurring in the eucaryotic genome and appear to have unusual structures which may be involved in genetic regulation and in chromosome replication. In order to help elucidate these structures, we have investigated the complexes formed by the homopurine d(GA)4 and homopyrimidine d(TC)4 DNA oligonucleotides using 1 and 2 dimensional 1H NMR spectroscopy. We have identified at least four different structures formed by these DNAs: a B-DNA duplex, a triplex composed of one homopurine and two homopyrimidine strands, another heterocomplex containing both strands, and a homocomplex of the d(GA)4 strand. We review data on the duplex and triplex and present new information on a d(AG)4 self-complex.
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