Promoter deletions and essential for transformation of lettuce by the T-cyt gene: the phenotypes of transgenic plants
1999
Curtis, I.S. | He, C. | Jordi, W.J.R.M. | Davelaar, E. | Power, J.B. | Laat, A.M.M. de | Davey, M.R.
The Agrobacterium T-cyt gene was transferred into lettuce, Latuca sativa 'Saladin' using a genotype-independent transformation procedure employing a supervirulent Agrobacterium tumefaciens strain carrying the binary vector pMOG23. Kanamycin-resistant shoots were initiated from inoculated explants only when sites were deleted within the T-cyt promoter. In culture, kanamycin-resistant shoots exhibited several phenotypes, including gall production, reduced internode length (dwarfism) and vitrification together with differences in rooting. Rooted, neomycin phosphotransferase (NPTII)-positive plants recovered from their abnormal phenotype and self pollinated to produce viable seed, following transfer to the glasshouse. Kanamycin-resistant lettuce plants exhibited increased cytokinin and chlorophyll contents compared to non-transformed plants, physiological features which could benefit long-term storage of this leafy vegetable.
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