Formation of raphanusanins depends on extraction procedure and solvent
1998
Matsuoka, H. | Toda, Y. | Yoneyama, K. | Uda, Y.
Formation of raphanusanin, 3-[alpha-methoxy-(alpha-methylthio)methyl]-2-thioxopyrrolidine, which had been reported as a light-induced plant growth inhibitor in roots and seedlings of radish, was examined using different extraction procedures and solvents including methanol-d4. Both (3R*, 6R*)- and (3R*, 6S*)-isomers of the compound were detected in the methanol extract of autolysed radish roots, but not in the acetone nor in the water extracts. When autolysed radish seedlings were extracted with methanol-d4, raphanusanin-d3 having a CD3 group was formed instead of raphanusanin. In the case of blanched seedlings and roots, raphanusanins were detected only in the methanol extract of myrosinase-treated homogenates. These results demonstrate that raphantin or raphanusanin is an artifact formed during extraction of radish with methanol, and therefore it may not be involved in phototropism of radish.
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