In vitro effect of the red alga Hydropuntia cornea (J. Agardh) on the respiratory burst activity of sole (Solea senegalensis, Kaup 1858) phagocytes
2007
Díaz Rosales, Patricia | Felices, Carmen | Abdala, Roberto | Figueroa, Félix L. | Gómez Pinchetti, Juan Luis | Moriñigo, Miguel A. | Balebona, Maria Carmen
The stimulatory effect of different extracts from the red alga Hydropuntia cornea (formerly Gracilaria cornea) on the respiratory burst activity of sole phagocytes was evaluated in vitro. Aqueous and ethanolic extracts and extracellular polysaccharidic fractions from algal cultures were assayed. Phagocytes, isolated from sole kidney, were incubated in vitro with different algal fractions at concentrations of 10, 5, 2 and 1 mg mL⁻¹. As a positive control, commercial β-glucan from the alga Euglena gracilis was used. The results obtained indicate that an ethanolic extract from H. cornea, at 10 mg mL⁻¹, is capable of enhancing superoxide anion production in sole phagocytes when the cells are incubated for 30 min with the bacterium P. damselae ssp. piscicida and the extract. On the other hand, the commercial β-glucan leads to a significant increase at highest concentration, 10 mg mL⁻¹, after 30 min of incubation.
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