Genetic Diversity of Biocontrol Strains of Pseudomonas fluorescens Producing 2, 4-diacetylphloroglucinol from Southern India
2012
Immanuel, J Ebenezar | Hemamalini, R. | Gnanamanickam, S. S.
Production of the antibiotic 2,4-diacetylphloroglucinol (DAPG) contributes to biological control activity of many beneficial strains of the bacterium Pseudomonas fluorescens. The DAPG is toxic to many organisms, and crop yield increases have been reported after application of DAPG-producing Pseudomonas fluorescens. Here the genotypic diversity of the efficient biocontrol strains of Pseudomonas fluorescens was investigated. Amplified ribosomal DNA restriction analysis (ARDRA), random amplified polymorphic DNA analysis (RAPD), and whole cell BOX PCR analysis were carried out to determine the genetic variability among DAPG-producing strains of Pseudomonas fluorescens assembled from rice (Oryza sativa) rhizosphere in Southern India. As the outcome of these studies, phlD restriction pattern can be used as a marker for ecological studies or when selecting phl+ Pseudomonas strains for specific biocontrol application.
显示更多 [+] 显示较少 [-]