The sulfate-reducing bacterial community of sulfide-rich water of the Ust’-Kachka resort spring, Perm Krai, Russia
2012
Pimenov, N. V. | Kuranov, G. V. | Bryukhanov, A. L. | Veslopolova, E. F. | Koryukina, I. P. | Maslov, Yu. N.
Microbiological investigation of the highly mineralized, sulfide-rich cold spring of the Ust’-Kachka resort was carried out. The total number and biomass of microbial cells were 50 × 10³ cell/mL and 15 μg/L, respectively. While the total microbial number was low, the sulfate reduction rate determined by the radioisotope method was relatively high (0.575 mg S L⁻¹ day⁻¹). An enrichment culture was obtained on Widdel medium. According to the results of cloning the 16S rRNA gene fragments with subsequent restriction analysis, the dominant organisms were group 6 sulfate-reducing bacteria (Desulfovibrio-Desulfomicrobium) and the microorganism exhibiting 99% similarity to the anaerobic haloalkaliphilic bacterium Halanaerobium hydrogeniformans.
显示更多 [+] 显示较少 [-]