Aqueous two-phase systems: A new approach for the determination of p-aminophenol
2011
Rodrigues, Guilherme Dias | Lemos, Leandro Rodrigues de | Patrício, Pamela da Rocha | Silva, Luis Henrique Mendes da | Silva, Maria do Carmo Hespanhol da
A new method has been developed for the spectrophotometric determination of p-aminophenol (PAP) in water, paracetamol formulations and human urine samples with a recovery rate between 94.9 and 101%. This method exploits an aqueous two-phase system (ATPS) liquid–liquid extraction technique with the reaction of PAP, sodium nitroprusside and hydroxylamine hydrochloride in pH 12.0, which produces the [Fe₂(CN)₁₀]¹⁰⁻ anion complex that spontaneously concentrates in the top phase of the ATPS (K[Fe2(CN)10]10−=97.7K. The ATPS does not require an organic solvent, which is a safer and cleaner liquid–liquid extraction technique for the determination of PAP. The linear range of detection was from 5.00 to 500μgkg⁻¹ (R≥0.9990; n=8) with a coefficient of variation of 2.11% (n=5). The method exhibited a detection limit of 2.40μgkg⁻¹ and a quantification limit of 8.00μgkg⁻¹. The ATPS method showed a recovery that ranged between 96.4 and 103% for the determination of PAP in natural water and wastewater samples, which was in excellent agreement with the results of the standard 4-aminoantipyrine method that was performed on the same samples.
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