Dedifferentiation of starch-storing cultured tobacco cells: effects of 2,4-dichlorophenoxy acetic acid on multiplication, starch content, organellar DNA content, and starch synthesis gene expression
2002
Miyazawa, Y. | Kutsuna, N. | Inada, N. | Kuroiwa, H. | Kuroiwa, T. | Yoshida, S.
The addition of the auxin 2,4-dichlorophenoxy acetic acid (2,4-D) to tobacco Bright-Yellow-2 (BY-2) cells cultured in 2,4-D-depleted medium induced dedifferentiation of starch-storing cells into dividing cells. The changes in cell growth, starch content, organellar DNA content, and the transcript levels for the ADP-glucose pyrophosphorylase gene during the dedifferentiation of starch-storing cells were examined. Cell proliferation, decrease in starch content, and increase in DNA content began from 12-18 h after 2,4-D application. RNA-gel blot analysis showed a decrease in the ADP-glucose pyrophosphorylase mRNA levels, within 6 h of the addition of 2,4-D. Furthermore, experiments using aphidicolin revealed that starch degradation is linked with cell division.
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