We isolated non-urea-producing sake yeasts from a FAS2-G3748A (G1250S) mutant using established positive-screening medium (containing canavanine, arginine and ornithine; CAO medium) after selection of FAS2 mutants with cerulenin. Varying levels of ethyl caproate production were observed due to spontaneous allelic recombination of the FAS2 heterozygotes on the CAO medium. The resulting double mutants exhibited favorable fermentation properties for sake brewing.
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书目信息
出版者
Elsevier Masson SAS
其它主题
Heterozygosity; Ethyl caproate; Cerulenin; Sake; Loss of heterozygosity; Ethyl carbamate; Car1
语言
英语
类型
Journal Article; Text
2024-02-28
2026-02-03
MODS