16S‐23S ribosomal RNA spacer regions of Acetobacter europaeus and A. xylinum, tRNA genes and antitermination sequences
1996
The 16S‐23S ribosomal RNA spacer regions of Acetobacter europaeus DSM 6160, A. xylinum NCIB 11664 and A. xyUnion CL27 were amplified by PCR. Specific PCR products were obtained from each strain and their nucleotide sequences determined. The spacer region of A. europaeus comprises 768 nucleotides (nt), that of A. xylinum 778 nt and that of A. xylinum CL27 759 nt. Genes encoding tRNAᴵˡᵉ and tRNAᴬˡᵃ were identified. Putative antitermination sequences were found between the tRNAᴬˡᵃ sequence and the 5′‐terminus of the 23S rRNA coding sequence. The boxA element has the nucleotide sequence TGCTCTTTGATA. Based on hybridization data of digested chromosomal DNA with spacer‐specific probes, the copy number of the rrn operons on the chromosome of Acetobacter strains is estimated to be four.
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