Structural studies of the O‐specific polysaccharide from Plesiomonas shigelloides strain CNCTC 113/92
2000
The structure of the O‐specific side chain of the lipopolysaccharide (LPS) of Plesiomonas shigelloides, strain CNCTC 113/92 has been investigated by NMR spectroscopy, matrix‐assisted laser desorption/ionization time of flight mass spectrometry and sugar and methylation analysis. It was concluded that the polysaccharide is composed of a hexasaccharide repeating unit with the following structure: in which d‐β‐d‐Hepp is dglycero‐β‐dmanno‐heptopyranose and 6d‐β‐d‐Hep is 6‐deoxy‐β‐dmanno‐heptopyranose. This structure represents a novel hexasaccharide repeating unit of bacterial O‐antigen that is characteristic and unique to the Plesiomonas shigelloides strain. Using the high‐resolution magic angle spinning technique, 1H‐NMR spectra were also obtained for the O‐polysaccharide components of isolated LPS and in their original form directly on the surface of bacterial cells.
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