Measurement of the content of limit-dextrinase in cereal flours
1992
Procedures for the quantitative extraction, activation, and assay of limit-dextrinase in cereal flours have been developed. Extraction and activation require incubation in buffer containing 20mM cysteine for at least 16 h or with 25mM dithiothreitol for 5 h. Activity is assayed with a soluble, dyed substrate (Red-Pullulan) or an insoluble, dyed, and cross-linked substrate (Azurine-CL-Pullulan) which is dispensed in tablet form (Limit-DextriZyme tablets).
显示更多 [+] 显示较少 [-]AGROVOC关键词
书目信息
Carbohydrate research
卷
227
页码
257
- 268
ISSN
0008-6215
出版者
Elsevier B.V.
语言
英语
类型
Journal Article; Text
2024-02-28
MODS