Standardisation of Gymnema sylvestre R. Br. with reference to gymnemagenin by high-performance thin-layer chromatography
2004
Puratchimani, V. | Jha, S.
A simple and reproducible HPTLC method for the determination of gymnemagenin (1) in Gymnema sylvestre has been developed. Components were separated on pre-coated silica gel 60 F254 plates with chloroform:methanol (9:1) and scanned using a densitometric scanner in the UV reflectance mode at 290 nm. Linearity of determination of 1 was observed in the range 4-10 microgram. The average percentage recovery of 1 from an extract was 99.09 +/- 0.29, and the content of 1 in leaves of the title plant was 1.61% (dry weight).
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书目信息
出版者
American Veterinary Medical Association
其它主题
Phytochemicals; Standardization; Ultraviolet-visible spectroscopy; Plant physiology and biochemistry; Gymnemic acid; Gymnema sylvestre; Reproducibility of results; Thin layer; Gymnemagenin; Agricultural products (non-food plant); Triterpenoid saponins
语言
英语
注释
2019-12-04
类型
Journal Article; Text
2024-02-28
MODS