Analysis of benomyl residues in commodities by enzyme immunoassay: extraction, conversion, and high-performance liquid chromatography validations
1996
Lavin, L. | Young, B.S. | Spittler, T.D.
Use of a simple acetone extraction scheme afforded complete conversion of benomyl residues in food commodities for immunoassay analysis. Preliminary investigation of various extraction/hydrolysis schemes revealed this to be the most advantageous, and it was optimized for sensitivity, speed and simplicity. Residues were assayed using Benomyl/Carbendazim Kits and the RaPID Assay System (Ohmicron Corp., Newtown, PA). Ohmicron's Food Prep Kits (for sample extract cleanup) were also evaluated. The optimized extraction scheme, with or without the Food Prep Kit, generated results comparable to those by HPLC analysis. For five crop matrices- mustard greens, Italian dandelions, parsley, apples and strawberries- with real field residues ranging from 0.062 to 1.2 ppm, the correlation of immunoassay versus HPLC, across the range of all measurable samples in the study, was r = 0.963, n = 18. Overall performance data demonstrated the ability of this immunoassay system to adequately extract and quantitate bound residues in field-treated commodities.
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