Establishment of a loop-mediated isothermal amplification (LAMP) detection method for genetically modified maize MON88017
2016
Zhen, Zhen | Zhang, Minghui | Yu, Yanbo | Gao, Xuejun | Zhu, Yanming | Yan, Yu | Zhang, Renren
In this study, we developed a visual and rapid assay for the detection of MON88017 maize using the LAMP method. The LAMP method was specific for MON88017 event and takes only 40 min and the LAMP assay sensitivity is about 40 copies, which is the same level as that of conventional PCR method. LAMP amplicons can directly be detected by naked-eye inspection after adding SYBR Green I. In summary, the LAMP method is visual, faster, and more sensitive and does not need special equipment compared to the traditional PCR technique, which makes it a very higher efficiency approach for field tests and fast screening of GMO crops, especially for on-site, large-scale testing purposes in the field.
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