Okra mucilage powder: a novel functional ingredient with antioxidant activity and antibacterial mode of action revealed by scanning and transmission electron microscopy
2020
Nampuak, Chatmanee | Tongkhao, Kullanart
Okra mucilage powder (OMP) was firstly reported its antibacterial mode of action observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The reconstituted OMP or okra mucilage solution (OMS) with the concentration at 120 mg/mL was analysed for its total phenolic contents, total flavonoid contents and antioxidant activity reported as 11.33 ± 0.265 mg gallic acid/100 mL, 5.06 ± 0.197 mg catechin/100 mL and 176.18 ± 12.180 µmol Trolox equivalent/100 mL, respectively. The OMS exhibited strong antibacterial activities on Bacillus cereus, Staphylococcus aureus and Listeria monocytogenes with more than 3 log reduction from bacterial inoculum. Nonetheless, mode of action of OMS studied via TEM revealed that cells treated with OMS showed broken cell walls, cell membrane separation and intracellular content leakage. SEM photography revealed furrowed bacterial cell wall surface for this OMS‐treated cells when compared with untreated cells. Overall, our data suggested that the reconstituted OMP offered the greatest functional properties as antioxidant properties and antibacterial activities, aiding to its future application as a potential novel functional ingredient with health‐promoting application.
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