Determination of trace amount for germanium (Ge) by atomic fluorescence spectrometry in rice (Oryza sativa L.)
2007
Jiang, S.L. | Shi, C.H. | Wu, J.G.
The quantification of germanium in rice (Oryza sativa L.) was studied by atomic fluorescence spectrometry (AFS). The optimum experimental conditions or the generation of germanium hydride were 360-V photomultiplier tube voltage, 80-mA hollow cathode lamp, 2.5% (w/v) KBH₄ in 0.5% KOH solution and 20% H₃PO₄ mixture with 5% H₂SO₄. With these experimental conditions, the correlation coefficient (R²) of calibration curve was up to 0.9992. As a convenient and sensitive calibration curve, it was proposed for the determination of trace amounts of germanium in brown and milled rice samples. The results showed that the content of germanium in brown rice was higher than that in milled rice. The means of the content of germanium were 4.98 and 18.10 ng/g in milled rice and brown rice, respectively. Rice is an important food for human nutrition because it supplies starch, protein and the majority of micronutrients to humans, particularly in Asia. So determination the content of Germanium in rice is important for human health. In this study, we developed the method of AFS which was suitable for determination rice grain. With this method, the content of Ge could be analyzed accurately in rice, so that the good materials would be selected form germplasm resources of rice, which could be used to study physiological and genetic mechanisms and enhance germanium in diets for human.
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