Detection of Trypanosoma evansi in whole blood of domestic animals by DNA amplification method
2013
Shyam, K.P. | Gupta, S.K. | Singh, Ajit | Chaudhary, S.S. | Gupta, Jay Prakash
Trypanosoma evansi, a blood protozoan parasite causing ‘surra’ in domestic animals is widely prevalent in different parts and is of significant economic importance in livestock production. Parasitaemia is often intermittent and is not always possible to demonstrate the parasites in blood and hence diagnosis of trypanosomosis is often difficult. The present investigation has been carried out with the aim of detecting T. evansi in cattle, buffaloes and equines in the state of Haryana by parasitological (WBF), and DNA-detecting (TE-PCR) tests. Out of 205 field blood samples tested, only 2% were positive for T. evansi by WBF while PCR using synthetic oligonulceotide primers (21 mer sense and 22 mer antisense) targeted to a repetitive nuclear DNA sequence of T. evansi detected 60.49% samples positive. Study revealed TE-PCR to be highly sensitive to the conventional parasitological method and could be used for early of diagnosis of trypanosomosis in domestic animals.
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