Influence of avocado (Persea americana) Cx-cellulase on the structural features of avocado cellulose
1994
O'Donoghue, E.M. | Huber, D.J. | Timpa, J.D. | Erdos, G.W. | Brecht, J.K.
Avocado (Persea americana Mill.) fruit produce copious quantities of the enzyme Cx-cellulase (EC 3.2.1.4) during ripening. The possibility that Cx-cellulase is able to disrupt cellulose microfibril organization was investigated using molecular weight (Mr), X-ray diffraction, and ultrastructural analyses of cell walls from unripe avocado fruit incubated with the purified enzyme. Results indicate that Cx-cellulase causes a downshift in the Mr of unbranched cell-wall polymers in the Mr range of 10(6)-10(7) Da. There is an increase in the proportion of crystalline cellulose, and cellulose fibrils appear to lose cohesiveness in response to enzyme activity. We propose that Cx-cellulase attacks avocado cellulose at accessible sites in the peripheral and integral noncrystalline regions of the microfibril, resulting in a loss of cohesiveness within the fibril structure and an alteration in the binding of associated cell-wall matrix polysaccharides. The initial loss of avocado mesocarp firmness during fruit ripening may be linked to the onset of Cx-cellulase activity.
显示更多 [+] 显示较少 [-]