Postprandial monocyte activation in response to meals with high and low glycemic loads in overweight women
2007
Motton, Deborah D. | Keim, Nancy L. | Tenorio, Fatima A. | Horn, William F. | Rutledge, John C.
BACKGROUND: Recent data show that atherosclerosis is initiated and perpetuated by inflammatory events. Activation of immune cells such as monocytes initiates inflammation, a key step in atherosclerosis. OBJECTIVE: We hypothesize that a high-glycemic load meal activates inflammatory cells, and that this is mediated by elevated circulating triacylglycerol-rich lipoproteins. DESIGN: Sixteen women [body mass index (in kg/m²): 25.7-29.6], aged 20-48 y, consumed meals with a high or a low glycemic load in a crossover fashion. Blood samples were collected before and up to 8 h after the meals. Samples were measured for glucose, insulin, triacylglycerols, and circulating cytokines, and expression of tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) was measured by flow cytometry. RESULTS: At 3.5 h after the test meals, we observed a significant increase in monocytes expressing TNF-α with both high-and low-glycemic load meals. Also, expression of IL-1β in monocytes tended to increase, but the change was not significant. The glycemic load of the meal did not influence circulating cytokines and had only a minimal effect on postprandial triacylglycerol concentrations despite marked postprandial changes in glycemia and circulating insulin concentrations. CONCLUSIONS: In the postprandial state, monocytes can be activated by both high-and low-glycemic load meals. The glycemic load of a single meal did not have a significant effect on the degree of activation of the monocytes in women who displayed only a modest increase in circulating triacylglycerols in response to test meals. Future studies should examine the effect of glycemic load in subjects who have a hyperlipemic response to dietary carbohydrate.
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