The objective of this work was to analyze oxidative metabolism in Mya arenaria. Total Fe content in M. arenaria collected in the German Wadden Sea was 1.9±0.7, 0.7±0.1 and 0.17±0.01nmol/mg fresh weight (FW), in digestive glands (DG), mantle and gills, respectively. Labile Fe pool, assessed by electronic paramagnetic resonance (EPR), was 146±10pmol/mg FW, and by the fluorescence method employing calcein it was 118±9pmol/mg FW. The lipid radical content in the DG, assessed by EPR, was 27±7pmol/mg FW, and the thiobarbituric reactive substances content amounted to 57±8pmol/mg FW. Ascorbyl radical (A) content, assessed by quantification of EPR signals, was 0.04±0.01pmol/mg FW, and the ascorbate content (AH⁻) was 478±12pmol/mg FW. The ratio A/AH⁻ was (8±1)x10⁻⁵ AU, suggesting a minimum oxidative stress even under physiological conditions, presumably depending on basal metabolic functions. The content of nitric oxide (NO), assessed by EPR, was 99±3pmol/mg FW. The generation rate of NO by nitric oxide synthase-like activity (NOS-like) was assayed as NO production detected by EPR in the presence of l-arginine and NADPH, and was 3.16±0.06pmol/(mgFWmin). The data presented here document the detectable presence of highly reactive species in M. arenaria.
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出版者
Springer-Verlag
其它主题
Animal physiology and biochemistry; Ascorbyl radicals; Aquatic sciences related; Gas production (biological)
语言
英语
注释
Includes references
类型
Journal Article; Text
2024-02-28
2026-02-03
MODS