Comparing methods of genetic manipulation in Bacillus subtilis for expression of recombinant enzyme: Replicative or integrative (CRISPR-Cas9) plasmid?
2019
The present study evaluated the stability of Bacillus subtilis strains transformed with a replicative or integrative plasmid (via CRISPR-Cas9) to express a recombinant phytase. Both transformation methods did not affect the growth of B. subtilis, but the stability of the construct and the enzymatic activity was reduced in the strain transformed with the replicative plasmid.
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书目信息
Journal of microbiological methods
卷
164
页码
105667
ISSN
0167-7012
出版者
Systematic and Applied Acarology Society
其它主题
Crispr-cas systems; Bacterial transformation; Crispr-cas9; Plasmid stability; Phytases
语言
英语
类型
Journal Article; Text
2024-02-28
MODS