Isolation, formulation and antagonistic activity of rhizobacteria toward the potato cyst nematode Globodera pallida
1992
In a screening program, 16 bacterial isolates out of 179 isolated from roots and cysts caused a significant reduction (> 25%) in Globodera pallida penetration of potato roots. Six of these isolates caused significant reductions in repeated greenhouse tests. Spray application of the bacteria in suspensions with 0.2% methyl cellulose increased adhesion of the bacteria on seed pieces and promoted antagonistic activity when compared to application in water suspensions. The antagonistic activity was shown to be directly correlated with the number of colony forming units (cfu) present on the tuber. The isolates Agrobacterium radiobacter and Bacillus sphaericus at densities of 9.7 X 10(8) and 3.16 X 10(9) cfu ml-1, respectively, caused significant reductions in root infection of 24-41% in repeated experiments.
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