Rapid detection of fumonisin B1 in corn-based food by competitive direct dipstick enzyme immunoassay/enzyme-linked immunofiltration assay with integrated negative control reaction
1995
Membrane-based competitive enzyme immunoassays (EIA) in dipstick and immunofiltration (ELIFA) format for the detection of fumonisin B1 (FB1) were developed. A nylon membrane was coated with anti-FB1 antibodies and with anti-horseradish peroxidase (HRP) antibodies. A FB1-HRP conjugated was used both as the labeled antigen for competitive assay of FB1 and for noncompetitive binding to the anti-HRP antibodies (negative control). Both tests had visual detection limits for FB1 in the range of 7.5-10 ng/mL (buffer solutions) and 40-60 ng/g (corn-based food samples). The tests were applied to corn-based food samples, and the results were compared with those of microtiter plate EIA and high-performance liquid chromatography. With assay times of 60 min (dipsticks) and 10 min (ELIFA), these rapid tests should be useful to qualitatively detected FB1 corn-based foods.
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