Comparison of immunoassay and gas chromatography methods for the detection of the mycotoxin deoxynivalenol in grain samples
1996
The deoxynivalenol (DON) concentration in grain samples of wheat and corn, inoculated with Fusarium graminearum in the field, was determined by gas chromatography and competitive direct (CD)-ELISA using monoclonal antibodies. The two methods gave similar results with no false positives or negatives. Based on analyses of 140 wheat and 83 corn samples, the correlation coefficient (r) between the two methods was 0.984 +/- 0.015 for wheat and 0.986 +/- 0.018 for corn (P = 0.0001 for both). The slopes for the regression analysis of the corn and wheat data were 1.012 +/- 0.019 and 1.099 +/- 0.017, respectively. If only lower concentrations were considered, the 28 corn samples with DON concentrations below 5 ppm yielded a slope of 1.251 +/- 0.109 (r = 0.914 +/- 0.063), and the 63 wheat samples with DON concentrations below 2 ppm produced a slope of 1.220 +/- 0.132 (r = 0.763 +/- 0.083).
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