Analysis of luminal fluid microenvironments in the reproductive tract is pivotal to elucidate embryo-maternal signaling mechanisms responsible for successful reproduction in mammals, including cattle. Besides facilitating production of an optimized medium for in vitro fertilization and embryo culture in assisted reproductive technologies, screening of luminal fluid constituents in the oviduct and uterus could also provide critical information for elucidation of mechanisms underlying developmental programming. A key issue in this type of research is the sampling of luminal fluids. In this review we discuss the sampling techniques available for bovine species, including a recent in situ technique developed with the Ghent device, which allows rapid recovery of measurable amounts of pure uterine luminal fluid with minimal disturbance to the donor animal.
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出版者
American Chemical Society
其它主题
Ghent device; Catheterization; Biomarkers; Luminal fluid; Female; Medical and veterinary equipment; Embryo (animal); Oviductal fluids; Specimen handling; Instrumentation; Validity; Animal breeding and genetics; Female fertility; Veterinary
语言
英语
注释
In the special issue: experimental models in animal reproduction: theoretical and practical approaches / edited by alireza fazeli and ann van soon.
Includes references
类型
Journal Article; Text
2024-02-28
2026-02-03
MODS