In vitro propagation of endangered lippia filifolia mart. and schauer ex schauer
2006
PEIXOTO, PAULO HENRIQUE PEREIRA | SALIMENA, FááTIMA REGINA GONççALVES | SANTOS, MARCELO DE OLIVEIRA | GARCIA, LEONARDO DA SILVA | PIERRE, PATRííCIA MARIA DE OLIVEIRA | VICCINI, LYDERSON FACIO | OTONI, WAGNER CAMPOS
This work describes an efficient micropropagation protocol of Lippia filifolia. Nodal segments cultivation in MS medium supplemented with 6-benzylaminopurine (4.5 μμM)/αα-naphthaleneacetic acid (NAA; 54 nM) induced multiple shoots (in average 27 shoots per explant). Elongated shoots were rooted with NAA (0.11 μμM) and they maintained ploidy level of the in vitro produced explants. The basic chromosome number were 2n==2x==24. Regenerated rooted shoots were successfully acclimatized under shading house conditions. This is the first report involving the establishment of a protocol for shoot multiplication and rooting for endangered L. filifolia, contributing for germplasm preservation of this species.
显示更多 [+] 显示较少 [-]