LDH nanoparticle adjuvant subunit vaccine induces an effective immune response for porcine epidemic diarrhea virus
2022
Shi, Danyi | Fan, Baochao | Sun, Bing | Zhou, Jinzhu | Zhao, Yongxiang | Guo, Rongli | Ma, Zengjun | Song, Tao | Fan, Huiying | Li, Jizong | Li, Li | Li, Bin
Porcine Epidemic Diarrhea (PED) is a highly contagious intestinal disease which mostly caused by Porcine Epidemic Diarrhea Virus (PEDV). The PED has caused huge economic losses to the pig industry all over the world and a valid PEDV vaccine is needed to prevent the infection. In this study, we constructed expression plasmid based on the spike (S) gene of the epidemic PEDV strain. The recombinant eukaryotic S (Se) and prokaryotic S (Sp) subunit proteins were expressed and purified as vaccine antigens. We designed a new subunit vaccine based on S proteins, adjuvanted with layered double hydroxide (LDH). The results indicated that the LDH adjuvanted subunit vaccines induced a better immune effect in terms of antibody level and cellular immune response. In conclusion, this study showed a new design of a PEDV subunit vaccine with nanotechnology and demonstrated the potential for its clinical application.
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