Proteomics revealed the effects of nucleic acid metabolism, transcription and translation on postharvest broccoli senescence under elevated O2 stress
2023
Zhang, Yuxiao | Chen, Ying | Guo, Yanyin | Ma, Yangli | Yang, Mei | Fu, Ruiqing | Sun, Yupeng | Qin, Jing
Nucleic acids (DNA and RNA) play crucial roles in guiding protein synthesis and plant resistance to biotic and abiotic stresses, but our current available understanding concerning on the role and function of nucleic acid metabolism, transcription and translation processes in postharvest fruit and vegetables remains largely unclear. In this study, an iTRAQ-based proteomics was used to investigate changes of protein abundance in postharvest broccoli under 40% O₂ + 5% CO₂, 20% O₂ + 5% CO₂ and 5% O₂ + 5% CO₂ treatments. A total of 128 differentially expressed proteins (fold change ≥ 1.2) were identified. High O₂ stress reduced the DNA level by down-regulating the expression of proteins involved in DNA replication and up-regulating the expression of proteins DNA degradation. The decrease of DNA content, inhibition of transcription and promotion of RNA degradation induced by high O₂ stress further led to the mass loss of RNA content in postharvest broccoli. Additionally, high O₂ stress suppressed RNA processing, transport and translation and activated the expression of 20 S and 26 S proteasome, thereby accelerating the decrease of protein levels in postharvest broccoli. The decrease of DNA and RNA content might be related to the early yellowing and senescence in postharvest broccoli. This study revealed that the effect and mechanism of high O₂ stress on DNA and RNA metabolism, transcription and translation processes, and the role of these metabolic pathway on senescence in postharvest broccoli.
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