A simplified, non-survival technique for the catheterization of the bile duct in rats
1996
Chan, O. | Martinic, G. | Stanley, K.
This paper describes a simple non-survival surgical technique for bile duct cannulation in rats, suitable for short-term experiments. Anaesthesia is induced by an intraperitoneal injection of sodium pentobarbitone and is maintained using a volatile anaesthetic administered via a nose cone. A midline abdominal incision is made. the duodenum and part of the small intestine are placed beside the abdomen in saline-moistened gauze and the bile duct is exposed and isolated from surrounding connective tissue. An opening is created in the connective tissue below the duct and a length of suture is threaded through this opening. A catheter is inserted into the bile duct. Once bile fills the catheter hub, the suture is used to ligate the catheter in place. Bile samples are collected directly from the catheter hub. Once collection is complete the animals are euthanised while under anaesthesia. Bile samples can be collected by this technique for 1-2 hours.
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