The use of dietary synthetic antioxidants at recommended levels does not alter rat immune cell eicosanoid production or hepatic vitamin E concentration
1997
Fritsche, K. | McGuire, S.
The objective of this study was to determine whether dietary synthetic antioxidants at recommended levels altered rat immune cell eicosanoid production and vitamin E concentration. Weanling female Sprague-Dawley rats were fed AIN-76A diets containing 20% by weight corn oil, with no synthetic antioxidant, or 0.02% of either tertiary-butylhydroquinone (TBHQ) or butylated hydroxytoluene (BHT). After 6 wk, rats were killed and samples of blood and spleen were removed for immune cell isolation. Livers were removed for subsequent vitamin E analysis by reverse-phase high performance liquid chromatography. Immune cells were co-incubated with and without calcium ionophore A23187(20 micromoles/L for 30 min at 37 degrees C. The amount of prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) in the immune cell supernatants was quantified by enzyme immunoassay. Neither basal nor ionophore-stimulated release of PGE2 and LTB4 were significantly different across diet treatment groups. Furthermore, liver total lipids, vitamin E and phospholipid and triglyceride fatty acyl profiles were not significantly altered by these dietary antioxidants. In summary, the inclusion of the synthetic antioxidants, TBHQ or BHT, into rodent diets at recommended levels did not significantly alter in vitro eicosanoid production by rat immune cells or hepatic vitamin E concentration.
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