Antimutagenic and radical scavenging activity of wheat bran
2009
Brindzová, L. | Zalibera, M. | Jakubík, T. | Mikulášová, M. | Takácsová, M. | Mošovská, S. | Rapta, P.
This study examined the mutagenic, antimutagenic and antioxidant activities of the DMSO extracts from the wheat bran. Wheat bran extracts showed no genotoxicity toward <i>Salmonella typhimurium</i> TA98, TA100 and TA102 with or without S9 mix (an external metabolic system). In addition, wheat bran extracts expressed a dose-depend inhibitory effect on the mutagenicity of promutagen aflatoxin B1 (AFB1), an indirect mutagen which requires metabolic activation, and 3-(5-nitro-2-furyl)acrylic acid (5-NFAA), 2-nitrofluorene (2NF) and hydrogen peroxide (H <sub>2</sub> O <sub>2</sub>), direct mutagens, in <i>Salmonella typhimurium</i> TA98, TA100 and TA102 strains. Significant total antioxidant capacity of wheat bran extract was found by two standard spectroscopic assays based on ABTS and DPPH reagents. A special attention was focused to the reactive radical scavenging capacity of bran extract as one of its antioxitant activities. Wheat bran extract possessed higher ability to scavenge oxygen- and carbon-centered reactive radicals generated by the thermal decomposition of K <sub>2</sub> S <sub>2</sub> O <sub>8</sub> than BHT (70 and 65% scavenged radicals, respectively) during the electron paramagnetic resonance (EPR)/spintrapping test. The total phenolic content of wheat bran samples expressed in gallic acid equivalent was 2.7 mg/g, total flavonoid content expressed in rutin equivalent was 70.8 μg/g and the most abundant phenolic acids established by GC-MS method were isoferulic (3-hydroxy-4-metoxycinnamic) and ferulic (4-hydroxy-3-metoxycinnamic) acid, sinapic, caffeic, <i>p</i> -coumaric and vanillic acids.
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