Chemotaxis behaviour of Steinernema carpocapsae in response to Galleria mellonella (L.) larvae infected by con- or hetero-specific entomopathogenic nematodes
2021
Fu, Yaqi | Wang, Wenwu | Chen, Chaoying | Shan, Shaojie | Wei, Xianqin | Liu, Yaohua | Shapiro-Ilan, David | Gu, Xinghui | Hu, Bin | Yoshiga, Toyoshi | Ruan, Weibin
Entomopathogenic nematodes (EPNs) infect insects and reproduce within the host cadaver. The nematodes use chemotaxis to assist in host-finding. However, the chemotaxis of nematode species in response to insects previously infected by con- and hetero-specific EPN in a sand context has rarely been reported. Our aim was to address the response of Steinernema carpocapsae All (Sc) to Galleria mellonella cadavers infected by con- or hetero-specific EPNs (Heterorhabditis bacteriophora HB1, S. feltiae SN, S. rarum 17 C&E) in sand via chemotaxis assays. Additionally, the effect of different time points post-infection was also evaluated. Results indicated that previously infected G. mellonella larvae repelled infective juveniles (IJs) of Sc (p < 0.05) regardless of the time point post-infection and EPN species (con- or hetero-specific) and had a lower chemotaxis index as compared to that of no larvae addition in both sides. Moreover, it was found that for each of four tested EPN species, EPNs infected insect cadavers released dimethyl disulphide, which was identified by solid-phase microextraction (SPME) coupled to gas chromatography–mass spectrometry (GC–MS). We found that dimethyl disulphide acted as a repellent to Sc infective juveniles and reached a significant effect at the level of 0.106 mol/L. Our findings could provide a better understanding of movement behaviour of EPN infective juveniles in response to insect resources in the presence of con- or hetero-specific EPNs.
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