Detection of hepatitis A virus RNA in oyster meat
1997
Cromeans, T.L. | Nainan, O.V. | Margolis, H.S.
Detection of low concentrations of viruses in shellfish is possible with nucleic acid amplification by PCR. Hepatitis A virus (HAV) has been detected in oyster meat by reverse transcription-PCR (RT-PCR). We developed a method to identify HAV RNA by RT-PCR of total RNA extracted from oyster meat contaminated by adsorption, bioaccumulation, or injection. With dot blot hybridization detection of amplicons from the RT-PCR, rapid screening of a large number of samples is feasible. As few as 8 PFU of HAV/g of oyster meat can be detected.
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书目信息
出版者
Blackwell Publishing Ltd
其它主题
Statistics & numerical data; Prevention & control; Hepatovirus; Hepatovirus a; Reverse transcriptase polymerase chain reaction; Oyster meat; Isolation & purification; Evaluation studies as topic; Dna primers; Hepatitis a virus; Hepatitis a; Base sequence; Rapid methods; Transmission; Gene amplification; Sensitivity and specificity; Viral
语言
英语
注释
2019-12-05
类型
Journal Article; Text
2024-02-28
MODS