Development and characterization of microsatellite markers in the coffee-family herb Ophiorrhiza japonica (Rubiaceae)
2014
Nakamura, Koh | Ma, Chia-Jou | Huang, Chiun-Jr | Ho, Meng-Jung | Yang, Hsun-An | Peng, Ching-I
Ophiorrhiza japonica (Rubiaceae) in Taiwan shows altitudinal morphological differentiation, suggesting altitudinal genetic differentiation. However, global warming and/or road building are possibly causing human-mediated altitudinal gene flow and genetic disturbance. To investigate this possibility, we developed the first nuclear microsatellite markers for the genus. Using the low-cost compound microsatellite marker technique, 17 markers were developed and nine markers were polymorphic. The number of alleles per polymorphic locus ranged from 2 to 13. At population level, observed (H ₒ ) and expected (H ₑ ) heterozygosities ranged 0.070–0.831 and 0.068–0.812, respectively. The polymorphic information content ranged 0.066–0.787. No null allele, significant deviation from Hardy–Weinberg equilibrium, or significant linkage disequilibrium was observed. The 17 marker loci were successfully PCR-amplified in three congeneric species except for three cases. The developed microsatellite markers will be used to investigate genetic structure and gene flow among O. japonica populations at different altitudes.
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