Low-melanin containing pullulan production from sugarcane bagasse hydrolysate by Aureobasidium pullulans in fermentations assisted by light-emitting diode
2017
Terán Hilares, Ruly | Orsi, Camila Ayres | Ahmed, Muhammad Ajaz | Marcelino, Paulo Franco | Menegatti, Carlos Renato | Silva, Silvio Silvério da | Santos, Júlio César dos
Pullulan is a polymer produced by Aureobasidium pullulans and the main bottleneck for its industrial production is the presence of melanin pigment. In this study, light-emitting diodes (LEDs) of different wavelengths were used to assist the fermentation process aiming to produce low-melanin containing pullulan by wild strain of A. pullulans LB83 with different carbon sources. Under white light using glucose-based medium, 11.75g.L−1 of pullulan with high melanin content (45.70UA540nm.g−1) was obtained, this production improved in process assisted by blue LED light, that resulted in 15.77g.L−1 of pullulan with reduced content of melanin (4.46UA540nm.g−1). By using sugarcane bagasse (SCB) hydrolysate as carbon source, similar concentration of pullulan (about 20g.L−1) was achieved using white and blue LED lights, with lower melanin contents in last. Use of LED light was found as a promising approach to assist biotechnological process for low-melanin containing pullulan production.
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