Improvement of Biomass Production by Chlorella sp. MJ 11/11 for Use as a Feedstock for Biodiesel
2015
Ghosh, Supratim | Roy, Shantonu | Das, Debabrata
Algal biomass is gaining importance for biofuel production as it is rich in lipids. It becomes more significant when biomass is produced by capturing atmospheric greenhouse gas, CO₂. In the present study, the effect of different physicochemical parameters were studied on the biomass and lipid productivity in Chlorella sp. MJ 11/11. The different parameters viz. initial pH, nitrate concentration, and phosphate concentration were optimized using single-parameter studies. The interactions between the parameters were determined statistically using the Box-Behnken design of optimization. The optimal values were decided by analyzing them with response surface methodology. The optimum levels of the parameters (pH 6.5, nitrate concentration 0.375 g L⁻¹, and phosphate concentration 0.375 mL L⁻¹) yielded a maximum biomass concentration of 1.26 g L⁻¹at a constant light intensity of 100 μmol m⁻² s⁻¹and temperature of 30 °C. The effect of CO₂concentration on the biomass production was also investigated and was found to be a maximum of 4 g L⁻¹at 5 % air-CO₂mixture (v/v). Maximum lipid content of 24.6 % (w/w) was observed at 2 % air-CO₂mixture (v/v). Fatty acid analyses of the obtained algal biomass suggested that they could be a suitable feedstock for biodiesel production.
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