Chromosomal location of genes controlling lactate dehydrogenase in rye, wheat, and barley
1992
Thiele, V. | Melz, G.
The chromosomal location of lactate dehydrogenease (LDH) genes in rye, barley, and wheat was investigated using the disomic standard addition sets of 'Chinese Spring'--'Imperial' (wheat-rye) and 'Chinese Spring'--'Betzes (wheat-barley) and the monosomic rye-wheat additions, respectively. Gel electrophoresis was performed on 5% polyacrylamide gels. Diagnostic bands for LDH isozymes were present only in zone 2. Genes controlling LDH isozymes in rye were found to be located on rye chromosome 4R (Ldh-R1 and Ldh-R2) and in barley on chromosome 4H(V) (Ldh-H1 and Ldh-H2). Because of the incomplete rye-wheat addition set, only one gene for LDH isozymes of wheat could be located (wheat chromosome 4B; Ldh-B1). In the three cereal species investigated, LDH genes are located generally on homoeologous group 4 chromosomes.
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